Microbiology of food and animal feed. Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens. Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination… PAS 015 The cord comprises a cable

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Microbiology of food and animal feed. Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens. Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination… PAS 015 The cord comprises a cable1 Scope This Technical Specification describes the identification of Shiga toxin producing Escherichia coli (STEC) by means of the detection of the following genes: a) the major virulence genes of STEC, stx and eae (References[2][3]); b) the genes associated with the serogroups O157, O111, O26, O103, and O145 (References[3][4]). In any case, when one or both of the stx genes is are detected, the isolation of the strain is attempted. The isolation of