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Binding/Wash Buffer For Ni-NTA/Ni-IDA,10 mM Imidazole pH 7.9~8.1 Primary Cells The RoSSA particle localizes toDescription Biofargo Binding & Wash Buffer for Ni NTA Ni IDA is a ready to use, precisely formulated buffer designed for His tag protein purification by immobilized metal affinity chromatography (IMAC). Containing 10 mM imidazole at pH 7. 98. 1, this buffer effectively reduces non specific protein binding while maintaining strong and selective interaction between His tagged proteins and Ni NTA or Ni IDA resins. Optimized for reliability and